Biotin-Streptavidin System

Biotin-Streptavidin System designates one of the strongest non-covalent molecular interactions known in biology, with streptavidin binding biotin with extraordinary affinity, making this system universally used as a molecular glue for capturing, detecting, and immobilising biotinylated molecules in diverse laboratory, diagnostic, and pharmaceutical applications.

The biopharmaceutical industry employs biotin-streptavidin systems extensively across research and development workflows. ELISA assays use biotinylated detection antibodies captured by streptavidin-enzyme conjugates enabling signal amplification. Flow cytometry employs streptavidin-fluorochrome conjugates detecting biotinylated antibodies staining cell surface markers. Western blot detection systems use streptavidin-horseradish peroxidase for sensitive protein detection. Surface plasmon resonance experiments immobilise biotinylated targets on streptavidin sensor chips. Proximity ligation assays utilise biotin-streptavidin for proximity-dependent signal generation. Cell selection uses streptavidin-magnetic bead capture of biotinylated surface markers. As the system's extraordinary affinity and versatility continue enabling diverse applications, biotin-streptavidin remains one of the most fundamental tools in pharmaceutical research.