Detection Assay Selectivity

Detection assay selectivity ensures analytical methods measure intended analytes without interference from related substances. Cross-reactivity assessment detects unintended compound detection. Chromatographic separation achieves selectivity through physical resolution. Antibody specificity provides biological selectivity. Mass spectrometry enables molecular selectivity through mass-based detection.

Method validation confirms selectivity through related substance testing. Regulatory guidance establishes selectivity requirements. Metabolite interference requires careful assessment. Regulatory submissions demonstrate selectivity justifying method appropriateness. Emerging technologies including immunoassay enhancements improve selectivity.