Fluorescence Polarisation Immunoassay

Fluorescence polarisation immunoassay employs fluorescence polarisation changes quantitating small-molecule drug concentrations and biomarker levels in pharmaceutical samples. Immunoassay principles employ antibodies recognising target molecules; fluorescent tracer binding displacement alters polarisation measurable by fluorimeters. Assay simplicity enables rapid results within 30 minutes facilitating point-of-care testing. Homogeneous assay formats eliminate washing steps reducing user technical skill requirements.

Pharmaceutical applications include therapeutic drug monitoring determining circulating drug concentrations guiding dose adjustment. Biomarker detection identifies disease-related proteins predicting treatment response and prognosis. Immunosuppressant drugs including cyclosporine and tacrolimus employ fluorescence polarisation monitoring guiding transplant recipient dosing. Multiplexed assay formats simultaneously quantitate multiple analytes from single samples. Regulatory acceptance of fluorescence polarisation immunoassay supports clinical laboratory implementation. Point-of-care applications expand testing accessibility beyond centralised laboratories.